10x takara taq buffer Search Results


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10x pcr enhancer buffer  (Thermo Fisher)
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reverse transcriptase buffer  (Agilent technologies)
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10x pbs buffer (ph 7.4 sterile, w/o ca)  (BioShop)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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10x long range pcr buffer mg2+ (qiagen)  (Qiagen)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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sds-tris-glycine (10x  (Cosmo Bio USA)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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dreamtaq green buffer 10x  (Thermo Fisher)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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10x coating solution concentrate  (R&D Systems)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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10x goldtaq buffer  (Millipore)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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10x taq buffer  (Thermo Fisher)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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10x pcr rxn buffer  (Thermo Fisher)
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Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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10x reaction buffer s  (PEQLAB)
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PEQLAB 10x reaction buffer s
Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope <t>(10x</t> objective).
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Image Search Results


Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope (10x objective).

Journal: Bio-protocol

Article Title: Immunofluorescent Staining of Claudin-2 in Cultured Kidney Tubular Cells

doi: 10.21769/BioProtoc.3678

Figure Lengend Snippet: Phase contrast images of live cells grown on cover glasses were obtained using a Nikon eclipse TS100 microscope (10x objective).

Article Snippet: Micro cover glasses, round, No. 1, 18 mm (VWR ® International, catalog number: CA-48380-046), sterilize coverglasses using autoclave Microscope slides (Fisherbrand, Fisher Scientific, catalog number: 12-550-15) Clear tissue culture-treated 12-well microplates, (Corning Costar ® , Millipore-Sigma, catalog number: CLS3513) Parafilm Paper towel Cell line: LLC-PK 1 kidney tubule epithelial cell line (European Collection of Authenticated Cell Cultures (Salisbury, UK), catalog number: 86121112) For cell culture: Dulbecco's Modified Eagle Medium (D-MEM), low glucose, with L-glutamine 110 mg/L sodium pyruvate (Thermo Fisher, Gibco, catalog number: 11885084) 10% Fetal Bovine Serum (FBS) (Thermo Fisher, Gibco, catalog number: 12483-020) 1% Penicillin-Streptomycin (Penicillin-Streptomycin, 100x, sterile-filtered, cell culture tested) (Millipore-Sigma Aldrich, catalog number: P-4333) Trypsin-EDTA (0.05% Trypsin with EDTA 4Na) (Thermo Fisher, Gibco, catalog number: 25300062) Phosphate buffered saline (PBS) (Thermo Fisher, Gibco, catalog number: 10010023) Methanol (BioShop Canada, catalog number: Met302), cool to -20 °C before using 10x PBS Buffer (pH 7.4 Sterile, w/o Ca, Mg) (BioShop Canada, catalog number: PBS415), open in hood to maintain sterility for storage Bovine serum albumin (BSA) (fraction V, heat shock isolation, > 98%) (BioShop Canada, catalog number: ALB001) Polyclonal rabbit anti-Claudin-2 antibody (Thermo Fisher Scientific, Invitrogen, catalog number: 28530), aliquot to avoid repeated thawing, store at -20 °C Fluorophore-labeled secondary antibody: Donkey anti-Rabbit IgG labelled with Alexa Fluor 488 (green) (Thermo Fischer Scientific, catalog number: A-21206) or Donkey anti-Rabbit IgG labelled with Alexa Fluor 555 (red) (Thermo Fischer Scientific, catalog number: A- A31572) 2,4-diamidino-2-phenylindole (DAPI) (Thermo Fischer Scientific, catalog number: D1306) Make a 5 mg/ml DAPI stock solution by dissolving the contents of one vial (10 mg) in 2 ml of deionized water.

Techniques: Microscopy